Coding

Part:BBa_K4165077:Design

Designed by: Mennatallah Mahmoud Mohamed Abdelzaher Turky   Group: iGEM22_CU_Egypt   (2022-09-30)


WAP-four disulphide core domain 10A serine protease inhibitor.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 49
    Illegal PstI site found at 198
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 49
    Illegal PstI site found at 198
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 49
    Illegal PstI site found at 198
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 49
    Illegal PstI site found at 198
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

this part was codon optimized for the expression in E.coli


Source

genomic sequence retrieved from literature

References